RIES

Research Institute for Electronic Science, Hokkaido University

北海道大学
電子科学研究所

LAST UPDATE 2018/10/26

  • 研究者氏名
    Researcher Name

    与那嶺雄介　Yusuke YONAMINE
    助教　Assistant Professor

  • 所属
    Professional Affiliation

    北海道大学電子科学研究所
    Research Institute for Electronic Science, Hokkaido University

    生命科学研究部門 生体分子デバイス研究分野
    Section of Biology and Life Sciences, Laboratory of Molecular Devices

  • 研究キーワード
    Research Keywords

    生体高分子
    有機合成
    ラマンイメージング
    Biopolymer
    Organic synthesis
    Raman imaging
研究テーマ
Research Subject
ラマン顕微鏡による藻類細胞の代謝追跡
Monitoring metabolism of algae cells by Raman microscopy

研究の背景 Background

現在、化石燃料に替わるエネルギー源として、光合成生物により生産されるバイオマスが注目されています。微細藻類は、光子変換効率が高く、季節によらず培養が可能で、農作地を必要としない、といった利点があり、バイオ燃料源として期待できます。

As an alternative to fossil fuels, biomass produced by photosynthetic organisms has drawn great attention as a carbon-neutral and sustainable feedstock. Microalgae offer great potential for producing biofuels because they have a high photon conversion efficiency and can be harvested all-year-round, providing a reliable and continuous supply of biofuels without competition for arable land.

研究の目標 Outcome

ラマン分光法は、生体サンプルに対して非侵襲的な測定手法であり、細胞の代謝活動を妨げることなく観察可能です。さらに、安定同位体ラベルと組み合わせることで、代謝の経時変化を追跡することも可能です。現在、微細藻類の光合成活性を、ラマン顕微鏡によって追跡する手法を開発しています。

By virtue of its inert detection of inherent molecular vibrations via Raman scattering, Raman microscopy is an effective tool for non-invasive monitoring of intracellular chemical activity. Raman spectroscopy can be made more effective by combining it with stable isotope labelling as it helps us obtain the metabolic flux via chronologic information. We aim to develop a method for monitoring photosynthetic activity of algal cells at single-cell resolution by Raman spectroscopy with stable-isotopes as a tracking probe.

研究図Research Figure

Fig.1. (a) Monitoring of algal photosynthetic activity with stable isotopes by Raman spectroscopy. (b) Optical (left) and Raman (right) images of algal cells containing 0% and 20% deuterated paramylons. Fig.2. (a) Monitoring of algal carotenoid production with stable-isotope 13C by Raman spectroscopy. (b) Raman spectra of 13C- (red) and 12C- (black) astaxanthin accumulated in algal cells. Fig.3. Concept of “cyborg cells”: endowing natural cells with artificial materials.

文献 / Publications

ChemBioChem, 18, 2063–2068 (2017). Phys. Chem. Chem. Phys., 18, 12576-12581 (2016). Phys. Chem. Chem. Phys., 17, 32122-32125 (2015). ACS Appl. Mater. Interfaces, 5, 374–379 (2013). Biomacromolecules, 13, 2952–2957 (2012). Chem. Lett., 39, 84-85 (2010).

研究者HP