Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology


LAST UPDATE 2023/08/08

  • 研究者氏名
    Researcher Name

    苗加彰 Akira NOGA
    特任助教 Specially-appointed Assistant Professor
  • 所属
    Professional Affiliation

    Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology

    Molecular bioscience
  • 研究キーワード
    Research Keywords

Research Subject
Mechanism that establishes nine-fold symmetry of centriole (ciliary basal body)

研究の背景 Background


Cilia are conserved organelles in eukaryotes that are involved in cellular motility and material transportation. This organelle has ‘9+2 structure’ a conserved structure characterized by nine peripheral microtubules. This structure originates from the centriole (basal body), consisting of nine triplet microtubules arranged rotationally. Although proteins responsible for establishing the nine-fold symmetry of the centriole has been identified, their precise functional roles remain largely unknow.

研究の目標 Outcome


Our aim is to elucidate the mechanism that establishes the nine-fold symmetrical structure of the centriole. To this end, we are carrying out the functional and structural analyses of centriolar proteins. This accomplishment will have a major impact on the field of cell biology and will advance the understanding of diseases such as cancer, microcephaly, and situs inversus because abnormalities in the centriole lead to these diseases.

研究図Research Figure

Fig.1. Model of centriole assembly. Our data suggested that the microtubule wall (blue) and the cartwheel (pink) can assemble independently. In the model, there is interaction between the microtubule wall and the cartwheel, and they can only assemble into the centriole if their sizes and symmetries are matched.

Fig.2. Filamentous structures (magenta) between cartwheel spokes. We identified these structures by both conventional (left images) and cryo-EM (middle and right images). These structures contribute to  establishment of the nine-fold symmetrical structure of the centriole. The upper images show the overall cross-sectional views of centrioles and the lower images show the magnified view of the boxed regions in the upper images. Bars: 100nm

文献 / Publications

Noga, A., Horii, M., Goto, Y., Toyooka, K., Ishikawa, T., Hirono, M. (2022) EMBO Journal 41: e104582 (2022)
Hilbert, M*., Noga, A*., Frey, D., Hamel, V., Guichard, … Gönczy, P., Hirono, M., Steinmetz, MO. Nature Cell Biology 18: 393 (2016) (* equal contribution)