Institute of Multidisciplinary Research for Advanced Materials, Tohoku University


LAST UPDATE 2021/05/08

  • 研究者氏名
    Researcher Name

    福山真央 Mao FUKUYAMA
    講師 Lecturer
  • 所属
    Professional Affiliation

    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

    マテリアル・計測ハイブリッド研究センター ナノ・マイクロ計測化学研究分野
    Materials-Measurement Hybrid Research Center, Nano/Micro Chemical Measurements
  • 研究キーワード
    Research Keywords

    Droplet microfluidics
    Lab on a chip
    Interface chemistry
Research Subject
Interface chemistry in micrometer scale for the miniaturization of analytical systems

研究の背景 Background

近年、生物学等の学術分野や、製薬、臨床検査などの産業分野では、多数(> 1万個)の微少量の生体試料を短時間で測定する技術が求められています。その解決法の一つとして、数センチメートル角の基板(マイクロ化学チップ)上にマイクロメートルサイズの流体チャネルを作製し、その中で生成した1 – 100マイクロメートルサイズの単分散な水滴(マイクロ水滴)を反応場として分析操作に利用する研究が多く報告されています。

High throughput assays of small volume samples have been required in various research and industrial fields such as biology, pharmacy and diagnostics. Recently, micrometer-sized water-in-oil droplets (microdroplets) formed in microfluidics has been paid much attention as a picolitter-sized chemical container and number of application to the high throughput assays, such as single cell analysis, single molecule detection and protein characterization have been reported.

研究の目標 Outcome


The objectives of the research are to acquire understandings of interfacial phenomena of microdroplets and to development of new microanalytical methods using microdroplets. Recently, we reported protein quantification and protein crystallization in microdroplets by using spontaneous emulsification at the interface of microdroplets. These methods will be applied to single cell analysis and investigation of small chemical phenomena such as crystal nucleation.

研究図Research Figure

Fig.1. (A) Microdroplet formation in a microfluidic device. (B) Microanalytical system by using microdroplets (Droplet microfluidics). Fig.2. Selective condensation of microdroplet contents by using spontaneous emulsification and its application to protein quantification. Fig.3. Protein crystallization in microdroplets.

文献 / Publications

“自然乳化を利用したマイクロ水滴内包物の選択的濃縮法”, 福山真央,火原彰秀, 分析化学, 65 (2), 57 (2016).

Anal. Chem. 87, 3562 (2015). Anal. Methods 7, 7128 (2015). Anal. Chem. in press (2017, DOI: 10.1021/acs.analchem.7b02062).