Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology


LAST UPDATE 2017/02/25

  • 研究者氏名
    Researcher Name

    石内俊一 Shun-ichi ISHIUCHI
    教授 Professor
  • 所属
    Professional Affiliation

    Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology

    Molecular functions
  • 研究キーワード
    Research Keywords

    Gas phase spectroscopy
    Multi-resonance laser spectroscopy
    Mass spectrometry
    Laser desorption technique
    Electro spray ionization technique
    Molecular clusters
Research Subject
Gas phase spectroscopy of biomolecules

研究の背景 Background


Biomolecules are flexible molecules which form several conformations, and their structures and functions strongly correlate with each other. To clarify the relationship between the flexible structures and functions is signify curious subject even for the fundamental molecular science. For the first step, we must know the precise structures of the flexible molecules, which is, however, difficult especially in solution phase at room temperature because of their flexibility. By using gas phase spectroscopic methodology, conformer-selected measurements are possible.

研究の目標 Outcome


In biomolecular system, there are many processes in which cetain proteins recognize small molecules and their functions are regulated. One of the typical such processes is neurotransmission. We apply laser desorption technique and electro spray method to catecholamine neurotransmitters and partial peptides of their receptor which recognize ligands to introduce them in the vacuum, and aim to clarify the structures of each molecules and complexes by using several laser spectroscopic techniques.

研究図Research Figure

Fig.1. Laser desorption supersonic jet technique Fig.2. UV spectrum of dopamine (bottom) and conformer-selected UV spectra (upper). Fig.3. β2-adrenaline receptor and SIVSF peptide which is a binding site with adrenaline

文献 / Publications

Chem. Phys., 445, 21-30 (2014). Phys. Chem. Chem. Phys., 15, 5163-5175 (2013). Phys. Chem. Chem. Phys., 15, 957-964 (2013). Chem. Phys., 419, 145-152 (2013). Chem. Lett., 42, 1166-1167 (2013). Phys. Chem. Chem. Phys., 13, 7812-7820 (2011). J. Phys. Chem. A, , 115, 10363-10369 (2011). J. Phys. Chem. Lett., 1, 1130-1133 (2010).