The Institute of Scientific and Industrial Research, Osaka University


LAST UPDATE 2017/02/26

  • 研究者氏名
    Researcher Name

    永井健治 Takeharu NAGAI
    教授 Professor
  • 所属
    Professional Affiliation

    The Institute of Scientific and Industrial Research, Osaka University

    Department of Biomolecular Science and Technology
  • 研究キーワード
    Research Keywords

    Luminescent proteins
    Minority biology
    Green biology
Research Subject
Development of biophotonics technology applicable to minority biology and green biology

研究の背景 Background


In order to develop technologies useful for not only drug discovery and tailor-made medicine but also sustainable earth environment, it is essential to comprehensively understand biological phenomena by getting information regarding spatiotemporal biomolecular reactions in live cell and whole organisms. In particular, strong interest in research which applies otogenetic manipulation in conjunction with bioimaging is growing rapidly.

研究の目標 Outcome


The main objective of the research is to figure out principles of how small number biomolecules can cooperatively function to lead to robust phenomena at macro scale level by means of molecular spies that could be made by engineering of fluorescent /chemiluminescent proteins and photosensitive proteins as well as nanoscopic optics that can operate and visualize the molecular spies. In addition, we aim at social implementation of the light-emitting plants (LEP) made by introduction of chemiluminescence-related genes into the plant chromosome, thereby realizing electrical power free light source.

研究図Research Figure

Fig.1. Multicolor fluorescence imaging of cellular compartments in live human cells. Nucleus, mitochondria, endoplasmic reticulum and plasma membrane were labeled Sirius, Venus, mseCFP and mCherry, respectively. Fig.2. We are constructing optical microscopies such as cavityenhanced absorption microscopy for biomolecular imaging without staining, and nanoscopy based on SPoD/ExPAN (super resolution by polarization demodulation/excitation polarization angle narrowing Fig.3. Light-emitting liverworts (Marchantia) that was genemodified by introduction of a gene encoding super-duper greenemitting luminescent protein based on hybrid of chemiluminescent protein and fluorescent protein to increase brightness and emission color.

文献 / Publications

化学発光を利用した生命機能の可視化 化学と生物 49, 555-559 (2011)、回折限界を超えた超解像蛍光顕微鏡 化学 69, 21-26 (2014) Nature Methods 7, 729-732 (2010), Science 333, 1888-1891 (2011), Nature Communications 3, 1262 (2012), Scientific Reports 3, 2629 (2013), ACS Chemical Biology 9, 1197-1203 (2014)