The Institute of Scientific and Industrial Research, Osaka University


LAST UPDATE 2017/02/26

  • 研究者氏名
    Researcher Name

    松田知己 Tomoki MATSUDA
    准教授 Associate Professor
  • 所属
    Professional Affiliation

    The Institute of Scientific and Industrial Research, Osaka University

    Division of Biological and Molecular Sciences・Department of Biomolecular Science and Engineering
  • 研究キーワード
    Research Keywords

    蛍光タンパク質 / 化学発光タンパク質
    Fluorescent protein / Chemiluminescent protein
    Protein engineering
Research Subject
Development of functional protein probes for bioimaging

研究の背景 Background


Living organisms are regulating their life activity by having functional relationship among variety of molecules and cells. Therefore, the elucidation of the functional connectivity between molecules and between cells is important to understand the life phenomena. In addition to this, in order to understand principle how biological functions exert, which leads drug discovery or tailor-made medicine, information about spatiotemporal dynamics of each biomolecule is also indispensable.

研究の目標 Outcome


We are developing genetically encoded functional indicators and new optical genetic tools based on Fölster resonance energy transfer (FRET) or Bioluminescence resonance energy transfer (BRET) by using fluorescent proteins or chemiluminescent proteins that enables to visualize spatiotemporal dynamics of biological phenomena at molecular level. Furthermore, by applying these new imaging tools we are going to approach for understanding of the biological phenomena from a systematic point of view.

研究図Research Figure

Fig.1. Photoactivation and imaging of spontaneous Ca2+ oscillation in rat hippocampal neuron dissociation culture by PA-TNXL Fig.2. Genetically encoded photosensitizer SuperNova, that enables disruption of adjacent proteins. Fig.3. Genetically encoded caged Ca2+ PACR, that enables reversible Ca2+ by irradiation of blue light.

文献 / Publications

Neuron, 87(4), 813 (2015). Nat. Methods, 12(6), 515 (2015). ACS Chem. Biol., 9(5), 1197 (2014). Front. Mol. Neurosci., 7, 90 (2014), Microscopy, 63(6), 403 (2014).
Sci. Rep., 3, 2629 (2013). ACS Chem. Neurosci., 4(6), 963 (2013). Sci. Rep., 3, 1398 (2013). J. Am. Chem. Soc., 135(1), 46 (2013)