CLS
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
東京工業大学
科学技術創成研究院
化学生命科学研究所

LAST UPDATE 2020/06/10
-
研究者氏名
Researcher Name朱博 Bo ZHU
助教 Assistant Professor -
所属
Professional Affiliation東京工業大学科学技術創成研究院化学生命科学研究所
分子生命化学領域
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
Molecular bioscience -
研究キーワード
Research Keywords蛋白質工学
高速分子進化工学
無細胞蛋白質合成
Protein engineering
In vitro directed evolution
Cell-free protein synthesis
- 研究テーマ
Research Subject -
分子ディスプレイ法を用いた機能性タンパク質のエンジニアリングと評価
Engineering and profiling of functional proteins via biomolecular display technologies
研究の背景 Background
The functional proteins, including enzymes, antibodies, regulatory factors, etc., play essential roles in living organisms. Many of these proteins have been or have the potential to be utilized in the chemical and food industry, medical diagnostics and treatment, and bioremediation. The biomolecular display technologies could serve as rapid and cost-efficient tools for better understanding or improving these functional proteins. The two powerful in vitro display technologies I have been working on have their unique advantages in protein/peptide-related researches. The mRNA display (Fig. 1) can handle a trillion-level library and do a great job in finding the novel targets of interest and describing the fitness
研究の目標 Outcome
A synergetic effect is expected when different display technologies are working in a consecutive or feedback manner. The primary focus of my current research is on developing a versatile research platform via a combination of mRNA/cDNA display and microbead display and its applications on enzyme engineering, novel enzyme discovery, fitness landscape profiling, and antibody-based fluoroimmunosensor construction.
研究図Research Figure

Fig.2. Schematic representation of microbead display. POI: protein of interest; FACS: fluorescence activated cell sorter.
文献 / Publications
PLoS ONE, 10, e0127479 (2015). J. Biosci. Bioeng.,117, 652 (2014). J. Biosci. Bioeng.,128, 290 (2019).Methods Mol. Biol., 1347, 87 (2015).
研究者HP
- zhu.b.ac
m.titech.ac.jp
- http://www.ueda.res.titech.ac.jp/