Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology


LAST UPDATE 2020/06/10

  • 研究者氏名
    Researcher Name

    朱博 Bo ZHU
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology

    Molecular bioscience
  • 研究キーワード
    Research Keywords

    Protein engineering
    In vitro directed evolution
    Cell-free protein synthesis
Research Subject
Engineering and profiling of functional proteins via biomolecular display technologies

研究の背景 Background

The functional proteins, including enzymes, antibodies, regulatory factors, etc., play essential roles in living organisms. Many of these proteins have been or have the potential to be utilized in the chemical and food industry, medical diagnostics and treatment, and bioremediation. The biomolecular display technologies could serve as rapid and cost-efficient tools for better understanding or improving these functional proteins. The two powerful in vitro display technologies I have been working on have their unique advantages in protein/peptide-related researches. The mRNA display (Fig. 1) can handle a trillion-level library and do a great job in finding the novel targets of interest and describing the fitness

研究の目標 Outcome

A synergetic effect is expected when different display technologies are working in a consecutive or feedback manner. The primary focus of my current research is on developing a versatile research platform via a combination of mRNA/cDNA display and microbead display and its applications on enzyme engineering, novel enzyme discovery, fitness landscape profiling, and antibody-based fluoroimmunosensor construction.

研究図Research Figure

Fig.1. Schematic representation of mRNA display. P in circle indicates a puromycin.

Fig.2. Schematic representation of microbead display. POI: protein of interest; FACS: fluorescence activated cell sorter.


文献 / Publications

PLoS ONE, 10, e0127479 (2015). J. Biosci. Bioeng.,117, 652 (2014). J. Biosci. Bioeng.,128, 290 (2019).Methods Mol. Biol., 1347, 87 (2015).