ISIR

The Institute of Scientific and Industrial Research, Osaka University

大阪大学
産業科学研究所

LAST UPDATE 2017/02/26

  • 研究者氏名
    Researcher Name

    川井清彦 Kiyohiko KAWAI
    准教授 Associate Professor
  • 所属
    Professional Affiliation

    大阪大学産業科学研究所
    The Institute of Scientific and Industrial Research, Osaka University

    励起分子化学研究分野
    Department of Molecular Excitation Chemistry
  • 研究キーワード
    Research Keywords

    修飾核酸の合成
    DNA、RNA検出
    1分子分析
    蛍光blinking
    Synthesis of modified oligonucleotides
    DNA and RNA detection
    Single molecule analysis
    Fluorescence blinking
研究テーマ
Research Subject
蛍光の blinking 観測に基づく 1 分子分析法の開発と生体高分子ダイナミクスの観測
Development of single-molecule analysis method based on fluorescence blinking and observation of biopolymer dynamics

研究の背景 Background

光を吸収し蛍光を放つ蛍光分子は、情報を読み分けるツールとして対象物質の標識に用いられ、病理臨床検査、遺伝子診断、生体イメージング、そして分子生物学の基礎研究のためのツールなど、様々な分野で活用されています。極微量の試料、究極的には1分子からの情報の読み出しを可能にする1分子蛍光検出法の開発が望まれています。

Fluorescent molecules are widely used as universal tools in molecular biology and diagnostic imaging. To achieve ultrasensitive analysis, ultimately, to extract information from a single biomolecule, development of detection method based on the observation of fluorescence at the single-molecule level is highly desired.

研究の目標 Outcome

蛍光分子1つ1つに着目した際に重要となる、初めて現れる蛍光分子の性質として、光の点滅挙動「blinking」に着目します。blinkingを理解し操ることにより、蛍光分子1分子から放たれる光を基に、生体高分子のダイナミクスとその機能解明を目指します。

We focus on a development of a detection method that relies on the properties of fluorescent molecules that become highlighted when we look at molecules at the single-molecule level. On the single-molecule level, fluorescent molecules often exhibit fluctuating emissions between bright ‘‘on’’ and dark “off” states, which is referred to as “blinking.” By understanding the factors that affect the blinking, we utileze single fluorescent molecules to investigate the correlation between dynamics and function of biomolecules.

研究図Research Figure

Fig.1. Detection of SNPs by monitoring blinking triggered by charge separation in DNA. Fig.2. Microenvironment around the fluorescent dye accessed by triplet blinking. Fig.3. Probing the DNA triplex by monitoring the blinking of Cy3 triggered by photo-isomerization.

文献 / Publications

Chem. Commun., 51, 4861 (2015); Chem. Commun., 50, 10478 (2014); J. Am. Chem. Soc., 134, 9406 (2012); J. Am. Chem. Soc., 134, 4806 (2012);
J. Am. Chem. Soc., 133, 15568 (2011); J. Am. Chem. Soc., 132, 14216 (2010); J. Am. Chem. Soc., 132, 627 (2010); Nature. Chem., 1, 156 (2009)

研究者HP