The Institute of Scientific and Industrial Research, Osaka University


LAST UPDATE 2017/02/26

  • 研究者氏名
    Researcher Name

    小野尭生 Takao ONO
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    The Institute of Scientific and Industrial Research, Osaka University

    Department of Semiconductor Electronics
  • 研究キーワード
    Research Keywords

    Biodevice, Lab on a chip, μ-TAS
    Microfabrication of polymer materials
    Single-molecule imaging, Single-molecule detection
    Carbon nanotube, Graphene
Research Subject
Biodevice for high-sensitive detection of biomolecules

研究の背景 Background


Biodevice is a promising tool for biochemical assay, clinical diagnostics and so on, due to its micro/nano structures. Downsizing the reaction volume and/or observation region improves the detection sensitivity for biomolecules and the measurement precision for biochemical reactions. It leads to the early diagnostics for diseases and the elucidation of the reaction mechanisms of biomolecules.

研究の目標 Outcome


Single-molecule detection device has been developed using an amorphous perfluoropolymer, which has unique optical properties. Microfabrication techniques for this material were established mainly using plasma processes. Detection and imaging of singlebiomolecule was carried out with the polymeric microwell array in sub-micron, attoliter scale. After joined ISIR in 2015, I will combine my biodevice with nanocarbon such as carbon nanotube and graphene, which has excellent electrical properties, and develop novel biodevice with higher sensitivity and functionality

研究図Research Figure

Fig.1. Anisotropic etching of amorphous perfluoropolymer. (Top) SEM image of the etched trenches. (Bottom) XPS C1s spectra from the trenches. Carbon-rich sidewall protection layer was spontaneously formed with the etching progress. Fig.2. Fluorescent detection of plasmainduced damage in the perfluoropolymer film. (Top) Stacked fluorescent images of the damaged film. (Bottom) The exponential distribution of the damage intensity, due to the exponential decay of the damaging species, VUV from plasma. Fig.3. Fluorescent detection of single enzyme molecule (β-galactosidase) using microdroplet array of 200 aL volume (2× 10-16 L). Single enzyme was stochastically encapsulated in a droplet and detected by its fluorescent product (fluorescein) which was highly accumulated in the droplet.

文献 / Publications

J. Photopolym. Sci. Technol., 27(3), 393 (2014). Trans. Mat. Res. Soc. Japan, 36(4), 553 (2011). J. Appl. Phys., 105, 013314 (2009). J. Photopolym. Sci. Technol., 22(5), 683 (2009).