Institute of Multidisciplinary Research for Advanced Materials, Tohoku University


LAST UPDATE 2022/08/30

  • 研究者氏名
    Researcher Name

    伊藤優志 Yuji ITOH
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

    有機・生命科学研究部門 生命分子ダイナミクス研究分野
    Division of Organic- and Bio-materials Research, Biological and Molecular Dynamics
  • 研究キーワード
    Research Keywords

    フェルスター共鳴エネルギー移動 (FRET)

    Protein dynamics
    Förster Resonance Energy Transfer (FRET)
    Single-molecule fluorescence measurement
Research Subject
Investigation of protein dynamics by single-molecule fluorescence measurements

研究の背景 Background

タンパク質は、生命の維持に於いて必須な生体高分子である。タンパク質は生体内で時間的・空間的に制御され、様々な重要な役割を果たしている。言い換えると、タンパク質は非常に「ダイナミックな」分子である。したがって、タンパク質の性質を理解するためには、その機能と構造に加えて、運動 (ダイナミクス) を理解することが重要である。タンパク質のダイナミクスを調べる強力な方法として、フェルスター共鳴エネルギー移動 (FRET) 測定や、蛍光相関分光法 (FCS) などが存在する。

Proteins are essential biomolecules to keep our lives. They are regulated spatiotemporally and play a variety of important roles in vivo. That is, proteins are highly “dynamic” molecules. Therefore, to understand the characteristics of proteins, it is of high importance to understand their dynamics as well as their functions and structures. There are some promising techniques to investigate the protein dynamics such as Förster Resonance Energy Transfer (FRET) measurement and Fluorescence Correlation Spectroscopy (FCS).

研究の目標 Outcome


The objective of our research is to investigate the protein dynamics by applying single-molecule fluorescence microscopy based on FRET measurements and FCS. By applying single-molecule measurements, we will extract information on each molecule bullied in ensemble measurements and will reveal the characteristics of proteins in detail. We will also propose some models on how proteins work in vivo based on the results. Finally, we aim to deeply understand the life phenomena at the molecular level.

研究図Research Figure

Fig.1. Optical setup for single-molecule FRET measurements.

Fig.2. Optical setup for FCS measurements.

文献 / Publications

Nucleic Acids Res., 49, 8642 (2021). Life Sci. Alliance, 4, e202001005 (2021). Nucleic Acids Res., 46, 7261 (2018). J. Mol. Biol. 428, 2916 (2016).