Institute for Materials Chemistry and Engineering, Kyushu University


LAST UPDATE 2017/02/25

  • 研究者氏名
    Researcher Name

    久保木タッサニーヤー Thasaneeya KUBOKI
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    Institute for Materials Chemistry and Engineering, Kyushu University

    Laboratory of Biomedical and Biophysical Chemistry
  • 研究キーワード
    Research Keywords

    Molecular Biology
    Genetic Engineering
    Cell migration
    Stem cell manipulation
Research Subject
Development of mechano-biomaterials for cell manipulation.

研究の背景 Background

Mechanobiology is an emerging research science that use the integrated knowledge from medical, engineer, cell biology and biomechanics fields to explain how the mechanical signal from cellular microenvironment could regulate the cell functions. We are seeking to understand the mechanism governing normal physiology/pathological conditions of the living cells by developing mechanical substrates to control the cell behaviors such as their directional migration, proliferation or differentiation. Elasticity tunable hydrogenous gelatin gel for cell manipulation is being developed using photocurable styrenated gelatin. The mechanism of cell-substrate interaction is being investigated using molecular biology and biochemistry approaches.

研究の目標 Outcome

In tissue engineering and regenerative medicine, stem cell is expected to be one of the potential sources for future cell based therapeutic strategies. Our research focuses on the analysis of MSC proteome on gels with different stiffness using 2 dimensional differential in gel analysis. We achieved in demonstrating the significant changes of the major cytoskeketon and other signaling proteins in respond to substrate elasticity. This finding provided significant impact on the designing of biomaterials to control stem cell fate. For the aspect of cell migration, we successfully fabricated the soft band patterned gels that induced a strong mechanorepellent effect on cell migration. This mechanical substrate is useful for the future development of cell separation devices based on the variability in the mechanical responsiveness of the different cell types.

研究図Research Figure

Fig.1. Proteins that changed their expressions in respond to the mechanical stimuli. Fig.2. Alteration of major cytoskeletons and focal adhesion protein on different substrate elasticity gelatinous hydrogels. Fig.3. Percentage of the cell response to narrow soft band gel suggesting a strong repelling migration at the early time of observation.

文献 / Publications

Cell Struct Funct. 2012;37(2):127-39, Langmuir 2014; 30, 6187-6196