Research Institute for Electronic Science, Hokkaido University


LAST UPDATE 2018/10/26

  • 研究者氏名
    Researcher Name

    与那嶺雄介 Yusuke YONAMINE
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    Research Institute for Electronic Science, Hokkaido University

    生命科学研究部門 生体分子デバイス研究分野
    Section of Biology and Life Sciences, Laboratory of Molecular Devices
  • 研究キーワード
    Research Keywords

    Organic synthesis
    Raman imaging
Research Subject
Monitoring metabolism of algae cells by Raman microscopy

研究の背景 Background


As an alternative to fossil fuels, biomass produced by photosynthetic organisms has drawn great attention as a carbon-neutral and sustainable feedstock. Microalgae offer great potential for producing biofuels because they have a high photon conversion efficiency and can be harvested all-year-round, providing a reliable and continuous supply of biofuels without competition for arable land.

研究の目標 Outcome


By virtue of its inert detection of inherent molecular vibrations via Raman scattering, Raman microscopy is an effective tool for non-invasive monitoring of intracellular chemical activity. Raman spectroscopy can be made more effective by combining it with stable isotope labelling as it helps us obtain the metabolic flux via chronologic information. We aim to develop a method for monitoring photosynthetic activity of algal cells at single-cell resolution by Raman spectroscopy with stable-isotopes as a tracking probe.

研究図Research Figure

Fig.1. (a) Monitoring of algal photosynthetic activity with stable isotopes by Raman spectroscopy. (b) Optical (left) and Raman (right) images of algal cells containing 0% and 20% deuterated paramylonsFig.2. (a) Monitoring of algal carotenoid production with stable-isotope 13C by Raman spectroscopy. (b) Raman spectra of 13C- (red) and 12C- (black) astaxanthin accumulated in algal cells. Fig.3. Concept of “cyborg cells”: endowing natural cells with artificial materials.

文献 / Publications

ChemBioChem, 18, 2063–2068 (2017). Phys. Chem. Chem. Phys., 18, 12576-12581 (2016). Phys. Chem. Chem. Phys., 17, 32122-32125 (2015). ACS Appl. Mater. Interfaces, 5, 374–379 (2013). Biomacromolecules, 13, 2952–2957 (2012). Chem. Lett., 39, 84-85 (2010).