Research Institute for Electronic Science, Hokkaido University


LAST UPDATE 2017/08/07

  • 研究者氏名
    Researcher Name

    石井宏和 Hirokazu ISHII
    特任助教 Specially Appointed Assistant Professor
  • 所属
    Professional Affiliation

    Research Institute for Electronic Science, Hokkaido University

    Laboratory of molecular and cellular biophysics
  • 研究キーワード
    Research Keywords

    Two photon microscopy
    Super resolution imaging
    Single molecule imaging
    Developmental Biology
Research Subject
超解像2光子顕微鏡の開発とin vivo深部イメージングへの展開
Development of super resolution two photon microscopy for in vivo deep imaging

研究の背景 Background

生命現象を理解するための最も基本的なアプローチの一つは、からだを構成する組織や細胞、分子などを顕微鏡で直接観察することです。特に、動的で複雑な生命現象を「生きたままの状態」で「鮮明」に観察することが求められています。多光子顕微鏡法は、からだの深部をin vivoで高解像度に観察できるため、脳神経科学などの分野で積極的に導入されてきました。

One of the most basic approaches to understanding the biological phenomena is to image the body components such as tissues, cells, and molecules by using microscopes. Especially, the microscope systems for in vivo and high-resolution imaging have been required because the biological phenomena is very dynamic and complexity. The two photon microscopy is a powerful system for in vivo high resolution deep imaging, and it has been used in neuroscience and so on.

研究の目標 Outcome

我々の研究室は最近、2光子STED(stimulated emission depletion)顕微鏡を開発し、固定した培養細胞の微細構造を「超」解像でイメージングすることに成功しました。私はこのシステムをさらに高度化して超解像in vivo深部イメージングを可能とし、生命現象の一端を解明することを目標にしています。

Recently, the two photon STED (stimulated emission depletion) microscope has been established in our laboratory. By using this microscope, we successfully observed the fine structures at “super” resolution in fixed cultured cells. My research purpose is to upgrade this microscope system to apply for in vivo deep imaging with super resolution and answer the biological questions.

研究図Research Figure

Fig.1. Our lab’s two-photon STED microscope system. Otomo et al., Microscopy 64, 227-236 (2015). Fig.2. Comparison of TPLSM (two-photon excitation laser scanning fluorescence microscopy) and TP-STED (two-photon STED microscopy). Otomo et al., Microscopy 64, 227-236 (2015).

文献 / Publications

Dev. Growth Differ. 56, 175-188 (2014), PLoS ONE 8, e52996 (2013).