Research Institute for Electronic Science, Hokkaido University


LAST UPDATE 2018/04/19

  • 研究者氏名
    Researcher Name

    榎木亮介 Ryosuke ENOKI
    准教授 Associate Professor
  • 所属
    Professional Affiliation

    Research Institute for Electronic Science, Hokkaido University

    Cellular and Molecular Biophysics
  • 研究キーワード
    Research Keywords

    Circadian Rhythm/Biological Rhythm
    Optical Imaging
    Calcium Ion
    Neuronal Network
Research Subject
Optical Monitoring of Neuronal Network in the Master Circadian Clock

研究の背景 Background


In mammals, most physiological and behavioral events are subjected to well-controlled daily oscillation, and these rhythms are controlled by the hypothalamic suprachiasmatic nucleus (SCN) in the brain. Recent studies revealed that the SCN is a hierarchical and multi-oscillator system in which the neuronal network plays a critical role in expressing robust and coherent rhythms in physiology and behavior. To understand the mechanisms of the circadian clock at network level, one needs to visualize the SCN network at high resolution spatially and temporally.

研究の目標 Outcome


We developed a time-lapse fluorescence imaging system composed of a Nipkow-spinning disk confocal microscope and high sensitive CCD camera. Using various genetically encoded sensors, we are trying to visualize the spatial and temporal patterns of circadian rhythms in intracellular calcium, clock gene product, and membrane potential in a large population of neurons in the SCN.

研究図Research Figure

Fig.1. (top) Drawing of a mouse brain. SCN is located in the hypothalamus which receives photic input from the retina. (bottom) SCN sends major output signals to the PVN. Fig.2. (top) Expression pattern of calcium-sensitive probe, GCaMP6s, in the SCN and PVN. (bottom) Line-scan image of the calcium signals across the SCN and PVN over 8 days. Fig.3. Multifunctional recording of circadian rhythms in the SCN. Images of calcium, clock gene, neuronal firing, and membrane potential recordings are shown.

文献 / Publications

Ono et al., PNAS, 201613374, 2017. Enoki et al., PNAS, 2017. Enoki et al, Scientific Reports, 7:41733. 2017. Enoki et al., PNAS, 109(52):21498-503. 2012. Enoki et al., Neuron, 62.242-253, 2008.