Research Institute for Electronic Science, Hokkaido University


LAST UPDATE 2017/02/25

  • 研究者氏名
    Researcher Name

    根本知己 Tomomi NEMOTO
    教授 Professor
  • 所属
    Professional Affiliation

    Research Institute for Electronic Science, Hokkaido University

    Laboratory of molecular and cellular biophysics, Laboratory of molecular and cellular biophysics
  • 研究キーワード
    Research Keywords

    2 光子顕微鏡
    Two-photon microscopy
    Super resolution microscopy
    Neural activity and secretion
Research Subject
新規光学顕微鏡の開発による生体 in vivo イメージングの高度化と細胞生理学
Sophistication in in vivo imaging of living specimen by novel light microscopy and cell physiology

研究の背景 Background


For understanding the emergence of biological functions and their underlying mechanisms, it is critical to visualize dynamics of cells or biomolecules under a physiological condition – “in vivo”. We have successfully developed in vivo two-photon microcopy, which enables to observe neurons in the dentate gyrus at ~1.6 mm depth from the brain surface. Moreover, in order to visualize intracellular events and biomolecule complexes, we have developed a super-resolution microscopy by taking advantages of a novel laser light “vector beam”.

研究の目標 Outcome


We would like to create a new interdisciplinary field of natural sciences both by advancing “in vivo” imaging with novel laser and optics technologies as well as by investigating molecular cell physiology on neural and secretary activities. We hope that our researches promote national health and welfare by applying these technologies on new visualization analyses for connectomics on brains and neural circuits, physiological functions of endocrine and exocrine glands, and diseases including cancer and diabetes.

研究図Research Figure

Fig.1. in vivo imaging of cortex, hippocampal CA1 and detate gyrus (~1.6 mm from the surface) in living mouse brain by novel two-photon microcopy Fig.2. Morphological analysis of dendritic spine in fixed hippocampal neuron after treatment of novel clearing reagent Fig.3. Super-resolution imaging of microtubule network visualized by novel two-photon STED microscopy

文献 / Publications

Biomed. Opt. Express (2015) (in press), Anal. Sci. (2015) i(in press), PLoS ONE(2015) , 10(1): e0116280, BMC Bioinformatics (2015) (in press), Optics Express (2014), 22, 28215 Microscopy (2014), 64(1), 39, , Plant Morphology (2014) 24(1), 31, Int. J. Mol. Sci. (2014) 15(11), 19971, Opt. Express (2014), 22(5), 5746, Microscopy (2014) 63(1), 23