The Institute of Scientific and Industrial Research, Osaka University


LAST UPDATE 2017/02/26

  • 研究者氏名
    Researcher Name

    川井清彦 Kiyohiko KAWAI
    准教授 Associate Professor
  • 所属
    Professional Affiliation

    The Institute of Scientific and Industrial Research, Osaka University

    Department of Molecular Excitation Chemistry
  • 研究キーワード
    Research Keywords

    Synthesis of modified oligonucleotides
    DNA and RNA detection
    Single molecule analysis
    Fluorescence blinking
Research Subject
蛍光の blinking 観測に基づく 1 分子分析法の開発と生体高分子ダイナミクスの観測
Development of single-molecule analysis method based on fluorescence blinking and observation of biopolymer dynamics

研究の背景 Background


Fluorescent molecules are widely used as universal tools in molecular biology and diagnostic imaging. To achieve ultrasensitive analysis, ultimately, to extract information from a single biomolecule, development of detection method based on the observation of fluorescence at the single-molecule level is highly desired.

研究の目標 Outcome


We focus on a development of a detection method that relies on the properties of fluorescent molecules that become highlighted when we look at molecules at the single-molecule level. On the single-molecule level, fluorescent molecules often exhibit fluctuating emissions between bright ‘‘on’’ and dark “off” states, which is referred to as “blinking.” By understanding the factors that affect the blinking, we utileze single fluorescent molecules to investigate the correlation between dynamics and function of biomolecules.

研究図Research Figure

Fig.1. Detection of SNPs by monitoring blinking triggered by charge separation in DNA. Fig.2. Microenvironment around the fluorescent dye accessed by triplet blinking. Fig.3. Probing the DNA triplex by monitoring the blinking of Cy3 triggered by photo-isomerization.

文献 / Publications

Chem. Commun., 51, 4861 (2015); Chem. Commun., 50, 10478 (2014); J. Am. Chem. Soc., 134, 9406 (2012); J. Am. Chem. Soc., 134, 4806 (2012);
J. Am. Chem. Soc., 133, 15568 (2011); J. Am. Chem. Soc., 132, 14216 (2010); J. Am. Chem. Soc., 132, 627 (2010); Nature. Chem., 1, 156 (2009)