Institute of Multidisciplinary Research for Advanced Materials, Tohoku University


LAST UPDATE 2021/05/01

  • 研究者氏名
    Researcher Name

    小和田俊行 Toshiyuki KOWADA
    助教 Assistant Professor
  • 所属
    Professional Affiliation

    Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

    有機・生命科学研究部門 細胞機能分子化学研究分野
    Division of Organic- and Bio-materials Research, Cell Functional Molecular Chemistry
  • 研究キーワード
    Research Keywords

    Biofunctional Chemistry
    Functional Fluorescent Molecules
    Fluorescence Imaging
Research Subject
Visualization and manipulation of biological functions using functional small molecules

研究の背景 Background


Visualization of functions of living cells and biomolecules is highly useful for understanding complex biological systems. Moreover, the manipulation of the function of target biomolecules will help us to further understand them. Recently, imaging and controlling various biological functions using optical tools with high spatiotemporal resolution have been attracting more interest.

研究の目標 Outcome


The main objective of this research is to design and synthesize functional small molecules by utilizing the knowledge and techniques of physical chemistry and synthetic organic chemistry. Particularly, we aim to develop photoresponsive small molecules for the manipulation of biological functions, as well as fluorescent probes with detectability of biomolecules and environmental changes.

研究図Research Figure

Fig.1. (a) pH-activatable and chemically stable fluorescent probes pHocas-1–3 fixed on bone surface by forming tight bond to the bone matrix via bone-specific bisphosphonate ligands. (b) pHocas-AL with pHindependent fluorescence. (c) pH profile of fluorescence quantum yield. (d) Fluorescence reversibility of pHocas-3. Fig.2. (a) Two-photon time-lapse imaging of bone tissues, acquired at 5-min intervals for 8 h after injection of pHocas-3. White lines indicate cell borders. Scale bars, 20 μm. Excitation was at 940 nm. (b) Schematic representation of resorption-site movement.

文献 / Publications

J. Am. Chem. Soc. 2011, 133, 17772–17776. J. Clin. Invest. 2013, 123, 866–873. Optical Probes in Biology 2015, Chapter 13, 335–354. Chem. Soc. Rev. 2015, 44, 4953–4972. Chemical Biology 2016, 9(1), 3–6. Nat. Chem. Biol. 2016, 12, 579–585.