Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology


LAST UPDATE 2020/08/25

  • 研究者氏名
    Researcher Name

    大林龍胆 Ryudo OHBAYASHI
    特任助教 Specially appointed assistant professor
  • 所属
    Professional Affiliation

    Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology

    Molecular bioscience
  • 研究キーワード
    Research Keywords

    Cell cycle
    Cyanobacteria and Chloroplast
    Systems Biology
Research Subject
Elucidation and application of coordinated cell cycle control

研究の背景 Background


Bacterial cells maintain a constant genome amount and cell size by coordinating DNA replication and cell division with cell growth. Previously, most of the key factors involved in each proliferation step have been identified. On the other hand, however, the universal mechanisms of coordination of cell growth, DNA replication and cell division are largely unknown in bacteria. To understand the coordinated control of bacterial growth is important not only for basic science, but also for the application of microbes to material production.

研究の目標 Outcome


Using single-cell imaging and synthetic biology, we attempt to understand the coordinated regulation of cell growth in bacteria. And, in order to employ microorganisms as a cell factory, it is necessary to produce only useful materials without cell proliferation. Therefore, through the understanding of the mechanism of bacterial proliferation, we also aim to control the cell cycle by synthetic gene circuits and to create bacteria suitable for the production of various materials.

研究図Research Figure

Fig.1. Correlation between cell growth and chromosomal replication in cyanobacteria. Schematic diagrams showing changes in cell volume (top), gene copy number per cell (middle), and mRNA level per unit volume (bottom) Fig.2. Replication and transcription of multicopy chromosomes in cyanobacteria. Microscopic images of SYBR Green-stained (Chromosome), SSB-GFP (Replication), and RpoC-GFP (transcription) are shown.

Fig.3. Synthetic genetic circuit using repressilator (upper), and in vivo oscillation of each reporter protein (lower).

文献 / Publications

Front Microbiol. 11, 786 (2020). mBio 10, 2, 00510-19 (2019). Plant Cell Physiol 58, 2, 279-286 (2017). J Gen Appl Microbiol. 62, 3, 154-9 (2016). ISME J 10, 5, 1113-21 (2016). FEMS Microbiol Lett 344, 2 138-144 (2013)